Uppsala-Sweden/6 August 2009

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Digestion of ADH2(Z),pirB, pdc, PpetE, adh2(Y)

Digestion was performed using EcoRI and PstI from Fermentas with this protocol ([http://www.fermentas.com/fastdigest/index.html Protocol for Fast Digestion of Unpurified PCR Products with FastDigest® Enzymes)])

The calculated amounts for the digestion are written down here : Media:Digestion_06082009.xls


Purification of ADH2(Z),pirB, pdc, PpetE, adh2(Y)

Purification of the digestion mix was performed with Nucleo Spin® Extract 2 PCR cleanup protocol from Clontech

Purified product (50µl) in 1.5ml eppendorfs.

--Karl.brune 08:56, 10 August 2009 (UTC)

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