Team:Newcastle/Labwork/25 August 2009

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Contents 1 Stochastic Switch team 1.1 Summary 1.2 Restriction digest 1.2.1 Running the gel

Stochastic Switch team

Summary

Today we did restriction digest for a miniprep prepared for BBa_C0178. We also did minipreps for other six biobricks(....) from last week. We prepared DNa ladder to load to the gels. At the end of the day, we prepared our gel to run tomorrow.

Restriction digest

We followed the restriction digest protocol for our two miniprep prepared for BBa_C0178 and used the followings to prepare the solutions.

• H20, 6.5 ul
• 10x Buffer, 2ul
• DNA from minipreps, 10ul
• EcoRI, 0.5 ul
• PstI, 0.5 ul
• BSA, 0.5 ul (It was diluted 100 times)

We gave a quick pulse to mix everything in the tubes before we put them into 37C incubator for an hour.

Running the gel

Metal container team had already prepared the gel for us. We placed the gel tray into the gel electrophoresis device and top it up with 1xTAE buffer