EPF-Lausanne/25 August 2009

Wet Lab

Miniprep:

Made according to the protocol of Aisgen Miniprep Kit Elution in 50 ul. The RD2 #5 plasmids prep as some that have already been prepared will be sent for sequencing this afternoon.

Mediums

New medium were made to grow the double-transformed DH5 a (LovTap BB and RO2) -> should be without thy.

M9 was redone, pH adjusted with amino acid and filtred. for 1L:

5g glucose

6g Na2PO4

3g KH2PO4

1g NH4Cl

0.5g NaCl

0.12g MgSO4

0.01g CaCl2

Trp Operon synthesis

Using the Klenow fragments protocol and primers:£

Trp operon Rev

Trp operon Fwd

People in the lab

Nath, Basile, Gab, Christian