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Team:Cambridge/Project/Carotenoids/PR
From 2009.igem.org
Categories :
Project :
-
Overview
Sensitivity Tuner
--- Characterisation
--- Modelling
Colour Generators
--- Carotenoids (Orange/Red)
--- Melanin (Brown)
--- Violacein (Purple/Green)
The Future
Safety
Notebook :
Team Logistics :
Carotenoids
-
Introduction
Previous Research
Our Action Plan
Result Analysis
Reference
Previous Research
A few iGEM teams of previous years (e.g. Edinburgh 07, Edinburgh 08, Guelph 08) conducted research in the carotenoid system. We are fortunate to have access to some of their Biobricks in the registry. Here is a summary of the Biobricks available:
| Registry Code | Team | Sequence Description | Size |
|---|---|---|---|
| K118014 | Edinburgh 08 | rbs + CrtE | insert: 922bp, backbone: 2079bp, total: 3001bp. |
| K118006 | Edinburgh 08 | rbs + CrtB | insert: 949bp, backbone: 2079bp, total: 3028bp. |
| K118005 | Edinburgh 08 | rbs + CrtI | insert: 1498bp, backbone: 2079bp, total: 3577bp.
2 PstI sites removed. |
| K118013 | Edinburgh 08 | rbs + CrtY | insert: 1162bp, backbone: 2079bp, total: 3241bp. |
| K152005 | Guelph 08 | (rbs+CrtE) + (rbs+CrtB) + (rbs+CrtI)
+ (rbs+CrtY) + (rbs+GFP) | insert: 5412bp, backbone: 2079bp, total: 7491bp.
2 PstI sites in CrtI removed. |
| I742158 | Edinburgh 07 | rbs + CrtZ | insert: 547bp, backbone: 2079bp, total: 2626bp. |
The above enzymes all come from Pantoea ananatis (GenBank: D90087.2).
However, we have encountered certain issues during our preliminary research.
- In part K152005, the “CrtE” sequence (which originated from part K152001) contains 3 nucleotides different from the NCBI data base.
- Originally, the registry annotation for K152005 indicated that there was no ribosome binding site before gene CrtI (i.e. “…rbs+CrtB+CrtI+rbs+CrtY…”). After clarification with Team Guelph 08, we were told that it was solely an error in annotation. The registry entry for K152005 has been updated to the sequence in the table above.
- Teams of previous years constructed various composite parts containing two or more enzymes of the synthetic pathways (e.g. CrtEBI, CrtEBIY), but they were not available in the 2009 Distribution Plates.
