The E.coli didn´t grow in any of the plates again. =[ We discovered that the electrocompetent E.coli that we used yesterday were dead! So we transformed another electrocompetent E.coli using the rest of the ligation reactions we had.
We extremely hope to find colonies tomorrow!
Raíssa and Taís
ColiGuard
Minipreps from BBa_E0840, BBa_I718017, BBa_J61000 and BBa_I78016
Today we performed minipreps from yesterday's inoculated cultures, related to the biobricks already cited.
We followed protocol number 2, without modification (see Protocols section).
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