We tried to find the colonies containing the biobricks by colony PCR (using the forward primer of the insert and the reverse of the plasmid). We did 10 reactions of each biobrick. Unfortunately we didn´t find the correct bands.
GEL
Taís
We decided to make a bigger screening of our colonies to find the ones containing the biobricks (pJen1, pDLD, Lysozyme, JEN1 orf). We grew 24 colonies from each biobrick O.N. in liquid media.
"
