Team:UNICAMP-Brazil/Notebooks/August 5

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MicroGuards

Preparation of electrocompetent E. coli

  • Today we prepared electrocompetent E. coli to use in our transformations according to Protocol 4 (see protocols section).

Marcelo and Luige

YeastGuard

Yeast DNA extraction

  • Today we performed DNA extration from yeast Kluyveromyces lactis according to Protocol 5 (see protocols section). The agarose gel showed that the miniprep was successful, but we need to treat the DNA with RNAse.