Today we ran an agarose gel from the digested samples in order to assure that they were indeed digested. Unfourtunally, only BBa_I718017 and BBa_I718016 (Lox parts) were sucessful digested. Biobricks BBa_J61000 and BBa_E0840 appeared in the gel as a single band, reaching the approximately size of the backbone vector.
We repeated the digestion procedure for the biobricks that didn't work and, once more, we couldn't digest them!
We believe that those biobricks came without the insert! Only backbone vector is available...
Without those parts, it turns a lot more difficult to assemble our necessary devices =/
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