EPF-Lausanne/12 October 2009

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Wet Lab

Digestion assay (with Pst1 and SpeI) in order to check the double transformant -KO strand JRG1046.

We checked 3 clones of each double transformant: RO1.1+BB1 and RO2.4+BB1.

N.B: RO1.1+BB1 clone #1 culture wasn't pink at all

Took image of agarose gel:

RO1.1+BB1 #3 and 6 are double transformants:

( cut RO1 --> band of ~900bp, plasmid-900bp

RO2 --> band of 1.8 kb, plasmid - 1.8 kb

BB --> band of ~ 200bp, 400bp, plasmid - 600bp )

Started protocol to clone the "simple" LovTap into an iGEM plasmid: did the PCR on LovTap with primers that contained the iGEM prefix and suffix

Tú, Heidi