Back to Calendar
ColiGuard
finOP-pGEM digestion purification
- After confirming correctly finOP with pGEM ligations, today we gather all confirmed samples (for finO and for finP) into a single sample for each one.
- We ran 40 uL of each gathered sample in an agarose gel for later purification. Unfortunately, finP band appeared extremely weak in the gel and we weren't able to purify it. As for finO, it's band appeared in gel, but we decided to wait for finP in order to perform both purifications together.
- Thus, we took all the gathered finP sample left and concentrated it in speed vacuum, until ir reaches about 20 uL. Then we ran another agarose gel with this concentrated sample and with another 40 uL from finO gathered sample.
- This time, finP appeared as a barely visible band, but we were able to purify it, as we did for finO to.
- We performed purification using Invitrogen's Purelink Quick Gel Extractin Kit, following manufacturer's protocol (Protocol 7) without modifications).
Marcelo
|
|
|
|
"
