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Team:Brown/Notebook Meetings/9-11-09
From 2009.igem.org
9/11/09
iGEM Meeting
SFH 218
8:00 AM: Gary reminds us of our responsibility. We need to plan this semester! These next few weeks are the most important Adrian: Deadlines Jamboree is in 7 weeks! Wiki due date: 6 weeks. Adrian recommends we have it done in 5 weeks Presentation practice should be started in 5 weeks Data collection should be heavy these next few weeks Gary Judging is done by a criteria. Criteria is available to us. Regular meetings: Friday 8AM in SFH 218 Team 1 update Did not have any expression on the western blots Made new BL21 competent cells, however no transformations Test competence of cells End of next week goal: protein expression Team 2 update Made all of the constructs needed Agr-GFP ligated Trg-EnvZ Signal Pep-GFP Trouble is transforming into S. epi Looking for new vectors – talking to same guy that sent us PLM6 Primers designed for adapting to new vector Will’s computer program worked; designed for Tar He is concerned about mutagenesis – time consuming Submit for synthesis Will will pick 5 candidates Team 3 update We have OmC-RFP and Taz in pNoTat Ashley did stratagene, however her positive control plate did not work We can test with aspartate Will also test ribose binding protein If double transformation does not work, sequential transformation Everybody: come up with a week-by-week goal sheet. The team is meeting next Tuesday at 8AM.
