We digested the Adh1+Lysozyme/biofusion minipreps in order to connect it to the yeast expression vector (YEP). We used the XbaI and PstI enzymes. 9 of 10 digestions worked! =) The expected fragment size is 2000bp.
GEL
We chose two fragments and purified them from the agarose gel. We ligated them to YEP. We will transform it tomorrow.
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