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Team:Kyoto/CiC/Notebook/1012-1016
From 2009.igem.org
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
0914-0918 : what to do. what to do. what to do. what to do.
Monday, 14 August
To Do
- p-2-(1)(4)(7)(8)(10)
- Miniprep
- Restriction Enzyme Digestion
- p-2-(1)(4) E.X
- PCR purification kit
- p-2-(1)(7)(8)(10) E.S
- Gel extraction
- 4-(1)(2)(3)
- Colony PCR
- Insert check
Results
p-2-(1)(4)(7)(8)(10)
p-2-(1)(7)(8)(10) E.S
- Gel extraction kit
| NO. | Sample name | conc./(ng/ul) |
|---|---|---|
| 1 | p-2-(1) | 9.3 |
| 2 | p-2-(7) | 25.9 |
| 3 | p-2-(8) | 8.6 |
| 4 | p-2-(10) | 7.5 |
4-(1)(2)(3)
- This result indicates that (1)a, (2)b and (3)a were inserted properly.
Tuesday, 15 September
To Do
Further to 14 Sept.
- p-2-(1)(4)(7)(8)(10)
- Miniprep
- Restriction Enzyme Digestion
- p-2-(1)(4) E.X
- PCR purification kit
- p-2-(1)(7)(8)(10) E.S
- Gel extraction
- 4-(1)(2)(3)
- Colony PCR
- Insert check
Wednesday, 16 September
To Do
- ligation
- transformation
Results
ligation
| NO. | Sample name | conc./(ng/ul) |
|---|---|---|
| 1 | 11(stu1) | 103.2 |
Transformation
No colony was observed (090917 10:00)
Thursday, 17 September
To Do
- Make repetitive sequence (MPR)
- Transformation(retry)
