Team:Kyoto/CiC/Notebook/1012-1016

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : what to do. what to do. what to do. what to do.


Monday, 14 August

To Do

  • p-2-(1)(4)(7)(8)(10)
    • Miniprep
    • Restriction Enzyme Digestion
  • p-2-(1)(4) E.X
    • PCR purification kit
  • p-2-(1)(7)(8)(10) E.S
    • Gel extraction
  • 4-(1)(2)(3)
    • Colony PCR
    • Insert check

Results

p-2-(1)(4)(7)(8)(10)
p-2-(1)(7)(8)(10) E.S
  • Gel extraction kit

Kyoto 0914 PCR.png

Miniprep product conc.
NO.Sample nameconc./(ng/ul)
1p-2-(1)9.3
2p-2-(7)25.9
3p-2-(8)8.6
4p-2-(10)7.5
4-(1)(2)(3)

Kyoto 0914 PCR 2.png

  • This result indicates that (1)a, (2)b and (3)a were inserted properly.



Tuesday, 15 September

To Do

Further to 14 Sept.

  • p-2-(1)(4)(7)(8)(10)
    • Miniprep
    • Restriction Enzyme Digestion
  • p-2-(1)(4) E.X
    • PCR purification kit
  • p-2-(1)(7)(8)(10) E.S
    • Gel extraction
  • 4-(1)(2)(3)
    • Colony PCR
    • Insert check



Wednesday, 16 September

To Do

    • ligation
    • transformation

Results

ligation
conc.
NO.Sample nameconc./(ng/ul)
111(stu1)103.2
Transformation

No colony was observed (090917 10:00) 



Thursday, 17 September

To Do

  • Make repetitive sequence (MPR)
  • Transformation(retry)