We sent the pDLD+lysozyme device to iGEM today. =)
Yeast experiments
There are plenty of yeast colonies in the pDLD+Lys and Adh1+Lys plates. =)
We did PCR with 30 yeast colonies transformed with Adh1+Lysozyme and pDLD+Lysozyme. Unfortunately none of the colonies had the correct vector =/
We have no time to repeat the transformations =( Our hope is that there has been a problem to the PCR reaction and that the parts are correctly connected to the YEP vector. So we decided to go on with the transformed yeasts we have.
At night we find plenty yeast colonies in the pDLD+YFP and Adh1+YFP plates.
Considering our lack of time we decided to collect a pool of yeast colonies for each construction and inoculate in liquid media. This strategy was adopted for both constructions (pDLD+YFP and Adh1+YFP). These pre inocula will be used for induction tests of Lysozyme tomorrow.
"
