I) Results for yeaterday's tranformation.
CBG = Cohesive, Bangalore Genei BBG = Blunt, Bangalore Genei CP = Cohesive, Promega PCR
Hence, DH5a Rocks...
Also, PCR works.
II) Inoculated a single colony of this to LB+KAN for plasmid isolation.
III) Yesterdays PCR gave us only 2 bands, other than the expected range.. :-(
Why?
--rerun gel and rerun pcr--
III) Ran a test PCR of a-Mating Factor, p3w13d
IV) Double RE digest of Lycopene Cyclase.
Ran a gel.
Great gel, expected result.
V) Gel Eluted.
kept for ligation.
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