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Team:Kyoto/CiC/Notebook/0928-1002
From 2009.igem.org
- 0907-0911
- constructing HIV-TAT-(LALAAAA)3-generator
- 0914-0918
- constructing HIV-TAT-(LALAAAA)3-generator
- 0921-0925
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 0928-1002
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1005-1009
- constructing sig-GFP generator,sig-EGFP generator(HeLa)
- 1012-1016
- Observation
- 1019-1023
- Observation
0928-1004 : constructing sig-GFP generator and sig-EGFP generator(HeLa)
Monday, 28 September
To Do
- sig-EGFP generator(HeLa)
- Restriction Enzyme Digestion(Hind3)
Results
They were unexpectedly digested by Hind3,the Restriction Enzyme which would not work if the signal sequence were inserted in the vector correctly.
Tuesday, 29 September
To Do
- sig-GFP generator
- PCR(the second PCR)
- sig-EGFP generator(HeLa)
- phosphorylation signal sequence primer
- annealing signal sequence primer
- Restriction Enzyme Digestion(EcoR1 + Xho1)
- ligation
- transformation
Results
No colony was observed (090930)
Wednesday, 30 September
To Do
- sig-GFP generator
- miniprep I712074(T7 promoter)
- Restriction Enzyme Digestion
- ligation
- transformation
- sig-EGFP generator(HeLa)
- ligation
- transformation
Results
Transformation
Too many colonies were observed that we could find no fignificant differences between the treated cluture the control. (091001)
Thursday, 01 October
To Do
No experiments were undertaken
Friday, 02 October
To Do
No experiments were undertaken
Saturday, 03 October
To Do
No experiments were undertaken
Saturday, 04 October
To Do
No experiments were undertaken
Sunday, 05 October
To Do
No experiments were undertaken
