I) Got Transformants. Inoculated them in Broth. Isolation Failed. x-(
II) Took a-MF >put a non specific PCR with Vf2 and Vr2
>Gel
>Got Band around 1.3
>Partial amp occurs.
>proceed with P2f P2r
>Same size amplified.
Conclusion-
PCR does not work to amplify BioBricks.
We have to amplify using TSS Protocol
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