Team:Warsaw/Calendar-Main/16 June 2009

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Isolation of BioBricks from 2009 Kit Plates

Monika, Marek


  • Resuspension of DNA from selected wells with 15ul of H2O
  • transformation of chemocompetent TG1 cells (prepared by Jarek) with 3ul of DNA solution
  • Plating on LB medium supplemented with apropriate antibiotic

  • Selected BioBricks:

  • GFP mut3b - E0040 from Kit Plate1 well 14K
  • Promoter araC regulated - R0080 from Kit Plate1 well 12E
  • promoter lacIQ - I14032 from Kit Plate 2 well 11P
  • mCherry - E1010 from Kit Plate 1 well 18F
  • {{WarNotebookEnd}}