With the product of the mini-prep played yesterday we performed a PCR to verify the correct ligation of the ……… part into the ……. plasmid. In this PCR we used primers that join in the plasmid (VFoward and VReverse) and a primer that join in the insert (…… reverse).
Wesley
ColiGuard
Cre-Recombinase - transformations results
Today we checked yesterday's plates and, unfortunately, all grown colonies showed a red coloration!
That means that the vector probably religated with it's own part (RFP device). This could be avoided by purification of the plasmid's digested band.
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