Team:Kyoto/CiC/Notebook/0914-0918

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0907-0911
constructing HIV-TAT-(LALAAAA)3-generator
0914-0918
constructing HIV-TAT-(LALAAAA)3-generator
0921-0925
constructing sig-GFP generator,sig-EGFP generator(HeLa)
0928-1002
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1005-1009
constructing sig-GFP generator,sig-EGFP generator(HeLa)
1012-1016
Observation
1019-1023
Observation

0914-0918 : constructing HIV-TAT-(LALAAAA)3-generator

Monday, 14 September

To Do

  • No experiments were undertaken

Tuesday, 15 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • Colony PCR

Results

Bands of expected size were observed.

Wednesday, 16 September

To Do

  • HIV-TAT-(LALAAAA)3+B0015(terminator)
    • miniprep
    • Restriction Enzyme Digestion(Xba1,Pst1)
    • ligation
  • I712074(T7promoter)
    • Restriction Enzyme Digestion(Spe1,Pst1)
    • ligation

Results

Transformation

Colony was observed (090917)　

Thursday, 17 September

To Do

  • HIV-TAT-(LALAAAA)3-generator
    • colony PCR

Results

  • Binds of expected size were observed.

Friday, 18 September

To Do

  • No experiments were undertaken

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