Team:Cambridge/Project/ME02

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Melanin Pigment


Design

Constructing the BioBrick

Biobrick

Our aim is to make the MelA gene into a biobrick as follows:

MelAbiobrick.jpg

In order to do so, we had to remove forbidden restriction sites within the gene using primers and the in-fusion PCR technique. Primers were ordered as shown by the image below, to add the prefix and suffix and change the PstI restriction site from CTGCAG to CTGCAA: MelA primer map.JPG The PCR's will be done in the following steps, in order to remove both sites and prepare the gene as a biobrick: MelA.png

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Registry Code Type Sequence Description / Notes Length
Reporter MelA. The gene (melA) codes for a tyrosinase which produces a dark brown pigment from L-tyrosine. Production of the pigment requires the addition of copper and L-tyrosine supplements (the copper acts as a cofactor for the gene product) but no other precursors. The BioBrick sequence includes the native ribosome binding site. 1844bp
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