Today we began the tests with the yeasts transformed with the following constructions: YEP+pDLD-Lysozyme and YEP+Adh1-Lysozyme.
The pre inocula made yesterday grew. We inoculated it in 50mL of YNB Glucose Ura- or YNB Ethanol Ura-. The wild type yeast is Ura- so we grew it on YNB Glucose Ura+. The inocula grew for 4 hours in 30ºC and 150rpm until the OD 1,3 before the induction with lactic acid.
We performed the induction 5 hours long. We mesured the OD every one hour to construct a growth curve. At the end of the 5 hours we pelleted the culture, colected the supernatant and freezed the pellet to analyze in SDS-PAGE tomorrow.
We also dripped drops of the supernatant in filter paper discs and placed them in a Lactococcus lactis plate. We hope to see inhibition zones surrounding some of the filters.
The Yeasts inoculated in YNB Ethanol media didn't grow enough to begin the induction until the end of the day.
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