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Team:Brown/Notebook Meetings/5-27-09
From 2009.igem.org
Brown iGEM 2009
Phone Conference
5/27/09
(Pacific Time)
- 8:20PM: Meeting starts after a lot of technical difficulties
- 8:22: Brad Lowell at Harvard does not have UCP2, Invitrogen may have it looked up UCPs on invitrogen
- What expression vectors has the past iGEM teams used?
- We need E. coli expression vectors
- 8:32 Ahmad, ask them for the UCP
- 8:29: Indu wrote a nicely worded email.
- 8:31: Picking holins (there’s 3000!)
- There are some in the registry
- UC Berkeley from last year used Holin
- Ahmad will ask them for a couple Holins
- 8:49: CCDB – stops cell growth – can be another redundant mechanism
- It is in the registry
- 8:56: All of the parts so far are in the registry, we need a unique part to submit to the registry
- 9:01: We need to schedule a meeting with the panel for when we get back!
- June 17th
- Email the professors
- It would be nice to have Gary Wessel email them.
- 9:08: Michael write an email out to the professors inviting them
- Have the draft proofread by the group
- Have Wessel forward it to everybody
- 9:15: Somebody else – research a tight repressor!!
- 9:17: Indu will follow up phone call the lab for the tick protein
- Second idea to the histamine binding protein – have something that also binds IgE antibodies
- This provides redundancy – both binding current histamine and stopping further secretion of histamine
- Link them with glycine bridge
- 9:26: Probiotic system: natural bacteria that secretes protein into bloodstream
- We have getting it into the system from MIT yogurt
- We have intestinal implantation BactoKidney
- How do we get the bifunctional protein secreted?
- 9:44: There is a lady named Edith that Will will email – she can help us on probiotic drug transport across organs
- Ashley, Eli, Will, Minoo can research probiotic
- 9:45: Indu found a paper on protein that binds IGE antibody – she will put it on the google group
- Somebody needs to research IGE
- Eli will put up a paper about packaging
- Will is emailing about drug delivery systems
- 9:46: Any paper that may be useful to either project will be put on the google groups
- 9:56: Before 8pm PST – everyone go on the google group and build on the research that’s already been done.
- 10:00: Secretion: Eli, Will, Ashley
- OK everyone just decide what to research on the google group.
- 10:01: We are meeting on SUNDAY MAY 31 SAME TIME (Monday for Minoo)
- 10:02: Put up minutes.
- 10:16: bye everybody
