We have designed four NEW functionalised linker-extension standard vectors. All together, 106 parts were generated all cloned into our NEW functionalised linker-extension standard vectors and forty-one were single coding, thirteen intermediates and forty-eight composite parts.
Four NEW linker-extension standard vectors are constructed.
The forty-one BioBrick coding sequences are prepared and are listed as (i) coiled-coil-forming segments, (ii) oligimerisation domains, (iii) functional domains and others.
Thirteen BioBricks with intermediate parts are constructed.
The forty-eight BioBricks as composites are prepared, which are used in (i) polypeptide manufacturing, (ii) polypeptide membranes, (iii) coiled-coil polyhedra, (iv) regulated assembly and (v) functional biopolymers.
Name | Type | Description | Length |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005] | Plasmid_Backbone | BB-NIC-II-HisN | 2261 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006] | Plasmid_Backbone | BB-NIC-II-NC | 2240 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007] | Plasmid_Backbone | BB-NIC-II-HisC | 2167 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008] | Plasmid_Backbone | BB-NIC-III-HisN | 2261 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245009 BBa_K245009] | DNA | RFC 37 Scar Sequence | 6 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021] | Coding | cYFP | 252 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022] | Coding | nYFP | 462 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024] | Coding | Z1 | 36 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025] | Coding | AUP | 36 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026] | Coding | TA | 63 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027] | Coding | TB | 63 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028] | Coding | TC | 63 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029] | Coding | T1W_DDD | 189 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030] | Coding | T1W_KKK | 189 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031] | Coding | T1W_EEE | 186 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038] | Coding | NZ - cc homodimer | 87 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039] | Coding | CZ | 90 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042] | Coding | VASP | 135 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046] | Coding | ccdB | 306 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048] | Intermediate | H1-P2 | 204 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049] | Intermediate | KSI-DP-P1 | 489 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245050 BBa_K245050] | Composite | GyrB-CutA1 | 996 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245051 BBa_K245051] | Composite | GyrB-SG-SG-CutA1 | 1002 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052] | Intermediate | GCN_P2 | 204 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053] | Intermediate | P4-H1 | 204 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054] | Intermediate | P2-H1 | 204 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055] | Intermediate | P6-H1 | 204 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056] | Intermediate | P3-P5 | 204 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057] | Intermediate | P4-P2 | 204 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245058 BBa_K245058] | Composite | KSI-DP-P6 | 489 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059] | Intermediate | LL37-ELST | 267 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060] | Intermediate | ELST-ELST-ELST | 462 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061] | Intermediate | ELST-ELST | 306 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245062 BBa_K245062] | Composite | P1-H1-P2 | 309 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245063 BBa_K245063] | Composite | KSI-DP-P1-H1-P2 | 699 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245064 BBa_K245064] | Composite | P1-BCR-P2 | 318 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245065 BBa_K245065] | Composite | P1-APH-P2 | 345 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245066 BBa_K245066] | Composite | P1-GCN-P2 | 309 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245067 BBa_K245067] | Composite | P3-P4-H1 | 309 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245068 BBa_K245068] | Composite | P1-P3-P5 | 309 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245069 BBa_K245069] | Composite | KSI-DP-P1-P3-P5 | 699 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245070 BBa_K245070] | Composite | P6-P4-P2 | 309 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245071 BBa_K245071] | Composite | KSI-DP-P6-P4-P2 | 699 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245072 BBa_K245072] | Composite | KSI-DP-LL37-ELST | 657 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245073 BBa_K245073] | Composite | KSI-DP-LL37-ELST-ELST-ELST-ELST | 1125 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245074 BBa_K245074] | Composite | KSI-DP-LL-37-ELST-GCN-ELST | 918 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245075 BBa_K245075] | Composite | KSI-DP-EEE | 576 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245076 BBa_K245076] | Composite | KSI_DP-LL37-ELST-P2-ELST | 918 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245077 BBa_K245077] | Composite | KSI-DP-LL37-ELST-P4-ELST | 918 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245078 BBa_K245078] | Composite | LL37-ELST-GCN-ELST | 528 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245079 BBa_K245079] | Composite | GCN-ELST | 255 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245080 BBa_K245080] | DNA | scar Xba/Spe NgoMIV | 12 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245081 BBa_K245081] | Composite | P2-ELST | 255 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245082 BBa_K245082] | Composite | P4-ELST | 255 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245083 BBa_K245083] | Composite | TA-P1 | 168 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245084 BBa_K245084] | Composite | TB-P3 | 168 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245085 BBa_K245085] | Composite | TC-P5 | 168 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245086 BBa_K245086] | Composite | P2-TA | 168 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245087 BBa_K245087] | Composite | P4-TB | 168 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245088 BBa_K245088] | Composite | TC-P5 | 168 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245089 BBa_K245089] | Composite | foldon-APH | 222 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245090 BBa_K245090] | Composite | foldon-BCR | 195 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245091 BBa_K245091] | Composite | p53-APH1 | 186 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245092 BBa_K245092] | Composite | p53-APH | 231 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245093 BBa_K245093] | Composite | p53-BCR | 204 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245094 BBa_K245094] | Composite | foldon-p53 | 177 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245095 BBa_K245095] | Composite | GyrB-p53 | 753 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096] | Intermediate | BCR-P2 | 213 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097] | Intermediate | APH-P2 | 240 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245098 BBa_K245098] | Composite | GyrB-foldon | 744 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245099 BBa_K245099] | Composite | APH-p53 | 231 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245100 BBa_K245100] | Composite | BCR-p53 | 204 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245101 BBa_K245101] | Composite | LL37-ELST-GCN-ELST-P1 | 633 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245102 BBa_K245102] | Composite | LL37-ELST-GCN-ELST-P2 | 633 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245105 BBa_K245105] | Composite | ELST-ELST-ELST-mNGF | 1137 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245106 BBa_K245106] | Composite | RADA-mNGF | 741 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245107 BBa_K245107] | Composite | LL37-ELST-GCN-ELST-mNGF | 1203 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245108 BBa_K245108] | Composite | KSI-DP-P2/33-LL37-ELST-GCN-ELST | 945 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245111 BBa_K245111] | Composite | P2-P6-H1 | 309 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245112 BBa_K245112] | Composite | P5-P2-H1 | 309 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113] | Coding | KSI-DP | 378 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114] | Coding | LL37 | 111 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115] | Coding | KSI-DP-P2/33 | 405 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116] | Coding | GyrB | 657 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245117 BBa_K245117] | Coding | GyrB-SG | 663 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245118 BBa_K245118] | Coding | P1 | 99 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245119 BBa_K245119] | Coding | P2 | 99 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245120 BBa_K245120] | Coding | P3 | 99 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245121 BBa_K245121] | Coding | P4 | 99 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245122 BBa_K245122] | Coding | P5 | 99 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245123 BBa_K245123] | Coding | P6 | 99 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245124 BBa_K245124] | Coding | P7 | 99 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125] | Coding | P8 | 99 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245126 BBa_K245126] | Coding | H1 | 99 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127] | Coding | GCN | 99 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128] | Coding | p53 | 90 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129] | Coding | CutA1 | 333 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245130 BBa_K245130] | Coding | T1W_AEA | 186 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131] | Coding | ELST | 150 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245132 BBa_K245132] | Coding | APH1 | 90 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245133 BBa_K245133] | Coding | APH | 135 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134] | Coding | BCR | 108 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245135 BBa_K245135] | Coding | APHE | 135 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139] | Coding | RADA | 66 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140] | Coding | mNGF | 669 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141] | Coding | foldon | 81 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245200 BBa_K245200] | Composite | P1-P4-H1 | 309 |