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Team:Brown/Notebook Meetings/8-10-09
From 2009.igem.org
iGEM Meeting 8-10-09
SFH 218
Attendees: Diana, Adrian
- Team 1 update
- Got EV131 into pNoTat
- Growing in competent BL21
- IPTG induction
- Last year Team Resistance did an IPTG induction
- Will do Western blot (Diana has 6-His antibody)
- Team 2 update
- Successfully cloned Agr operon promoter, moved into pGEM
- Digested and ligated into Biobrick vector
- Agr promoter + RFP growing on plates, will test quorum sensor
- Electroporation failed; might be due to Tet plates, or faulty electrocompetent cells
- Made Trg-EnvZ. Doing blue-white test
- However, maybe did not distribute X-gal and IPTG evenly
- Looger said he will get back this week about receptor
- Running out of S. epi expression vector
- Team 3 update
- Ran SDS PAGE on Taz1, too much noise on protein gel
- Tested mutagenic primers
- Will send off for sequencing
- Administrative stuff
- Booked hotels for the Jamboree
- We have 2 time slots at the Jamboree, may split up??
