Virginia Commonwealth/24 July 2009

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Virginia Commonwealth/20 July 2009 - 2009.igem.org

Virginia Commonwealth/20 July 2009

From 2009.igem.org

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==Monday 20 July 2009== ===Results=== * Results of transformation with pSB4C5 backbone in NEB 10 beta three days after transformation ** Control (+) on LB NEB 10 beta (not shocked) had Lawn growth ** Control (-) on Cm NEB 10 beta (not shocked) had No growth {| cellpadding="10" align="center" border="0.9" padding="5" |+ |- ! Promoter!! Reporter !! Resistance/Plate !! Growth Observation !! |- ! J23110 | E0240 || Cm || 62 colonies || |- ! J23110 | J06702 || Cm || 56 colonies || |- ! J23113 | E0240 || Cm || 75 colonies || |- ! J23113 | J06702 || Cm || 33 colonies || |- ! J23116 | E0240 || Cm || 41 colonies || |- ! J23116 | J06702 || Cm || 65 colonies || |- ! R0011 | E0240 || Cm || hundreds of colonies || |- ! R0011 | J06702 || Cm || 5 colonies || |- ! R0040 | E0240 || Cm || 20 colonies || |- ! R0040 | J06702 || Cm || 12 colonies || |- ! J23105 | E0240 || Cm || 26 colonies || |- ! J23101 | E0240 || Cm || 34 colonies || |} [[User:Trentay|Trentay]] 14:17, 20 July 2009 (UTC) ''Craig and Clay'' * The registry did confirm that the correct resistance for the limonene synthesis related parts is ampicillin. New parts will also be sent. ---- ===Tasks=== ''Maria and Afton'' * Pick colonies for overnight culture from transformations * Ligate J23106 and J23101 with backbone pSB4C5 and RET E0240 * Transform liagated parts into NEB 10 beta [[User:Trentay|Trentay]] 18:40, 20 July 2009 (UTC) ---- ====Wetlab==== *1 *2