Team:UNICAMP-Brazil/Notebooks/August 5

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Preparation of electrocompetent E. coli

Today we prepared electrocompetent E. coli to use in our transformations according to protocol 4 (see protocol section).

Marcelo and Luige

YeastGuard: Yeast DNA extraction

Today we performed DNA extration from yeast Kluyveromyces lactis according to protocol 5 (see protocol section). The agarose gel showed that the mini-prep was successful, but we need to treat the DNA with RNAse.