Team:Newcastle/Labwork/25 August 2009
From 2009.igem.org
Contents |
Stochastic Switch team
Summary
Today we did restriction digest for a miniprep prepared for BBa_C0178. We also did minipreps for other six biobricks(....) from last week. We prepared DNa ladder to load to the gels. At the end of the day, we prepared our gel to run tomorrow.
Restriction digest
We followed the restriction digest protocol for our two miniprep prepared for BBa_C0178 and used the followings to prepare the solutions.
- H20, 6.5 ul
- 10x Buffer, 2ul
- DNA from minipreps, 10ul
- EcoRI, 0.5 ul
- PstI, 0.5 ul
- BSA, 0.5 ul (It was diluted 100 times)
We gave a quick pulse to mix everything in the tubes before we put them into 37C incubator for an hour.
Running the gel
Metal container team had already prepared the gel for us. We placed the gel tray into the gel electrophoresis device and top it up with 1xTAE buffer
News
Events
- 20 – 21 June 2009 - Europe workshop (London)
- 23 – 24 June 2009 - UK iGEM meetup (Edinburgh)
- 23 October Practice Presentation (Newcastle)
- 23 October T-shirts are ready
- 27 October Practice Presentation (Sunderland)
- 27 October Poster is ready
- 30 October – 2 November 2009 - Jamboree (Boston)
Social Net
- Newcastle iGEM Twitter
- [http://www.facebook.com/home.php#/group.php?gid=131709337641 Newcastle on Facebook]
- [http://www.youtube.com/user/newcastle2009igem Newcastle Youtube Channel]