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EPF-Lausanne/25 August 2009
From 2009.igem.org
Wet Lab
Miniprep:
Made according to the protocol of Aisgen Miniprep Kit Elution in 50 ul. The RD2 #5 plasmids prep as some that have already been prepared will be sent for sequencing this afternoon.
Mediums
New medium were made to grow the double-transformed DH5 a (LovTap BB and RO2) -> should be without thy.
M9 was redone, pH adjusted with amino acid and filtred. for 1L:
- 5g glucose
- 6g Na2PO4
- 3g KH2PO4
- 1g NH4Cl
- 0.5g NaCl
- 0.12g MgSO4
- 0.01g CaCl2
Trp Operon synthesis
Using the Klenow fragments protocol and primers:£
- Trp operon Rev
- Trp operon Fwd
| 2x Trp | 1x Negative Control | ||
|---|---|---|---|
|
[http://partsregistry.org/Part:BBa_B0010 BBa_B0010] |
Terminator |
A |
13D (1) |
|
[http://partsregistry.org/Part:BBa_R0010 BBa_R0010] |
Promoter LacI |
A |
1D (1) |
|
[http://partsregistry.org/Part:BBa_B0030 BBa_B0030] |
RBS |
A |
1H (1) |
|
[http://partsregistry.org/Part:BBa_E0240 BBa_E0240] |
RBS-GFP-TER |
A |
12M (1) |
|
[http://partsregistry.org/Part:BBa_I13507 BBa_I13507] |
RBS-mRFP-TER |
A |
22O (1) |
|
[http://partsregistry.org/Part:BBa_J13002 BBa_J13002] |
pTetR-RBS |
A |
13B (1) |
People in the lab
Nath, Basile, Gab, Christian
