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Primer design
During the week from July 20th to 25th we designed all primers that we´re going to use in our project. All the sequences are showed on July 25th notebook.
Coliguard: Restriction Assay
After the recovery of F plasmid (July 16th), we performed a digestion of the mini-prep's product, in order to assure that we indeed recover the plasmid itself.
We did this assay by using two restriction enzymes: Eco RI and Cla I. We followed protocol nº X, without modifications.
We then compared the pattern provided us by pDRAW software with an eletrophoresis gel of the digestion product.
According to this assay, we indeed were able to recover the F Plasmid!
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Gabriel, Marcelo and Victor.
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