Team:UNICAMP-Brazil/Notebooks/September 10

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YeastGuard

New biobricks in biobrick format

In order to make the new biobricks, the new parts that were digested with the enzymes Xba1 and Spe1 must be ligated to the biofusion vector, that was also already digested with the same enzymes. So today we prepared the ligation reaction (according to Protocol X) that will be kept at 4ºC O/N.