Team:UNICAMP-Brazil/Notebooks/September 14

From 2009.igem.org

Revision as of 15:17, 14 September 2009 by Mbassalo (Talk | contribs)
(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Topo l2.gif topo_r_igem.gif
topo_r_b.gif
Back to Calendar

ColiGuard

finO and finP's transformed bacteria

  • All of yesterday's transformed and plated cells grew in the media!
  • We then selected 10 colonies of each transformation (finO+pSB1A3 and finP+pSB1A3) in order to confirm them. We inoculated those colonies separately into liquid LB-AMP media, and let then grow O/N at 37ºC.
  • We will then proceed to the miniprep's of the cultures and, once we could run and agarose gel of the extracted plamids, we will be able to confirm that both finO and finP are indeed in biobrick format.

Marcelo