Since all of our fragments are correctly digested, we started today the ligation of both finO and finP sequences to the vector pSB1A3. Each sequence was ligated singly to the vector and, only after each one is confirmed, we'll join then both to a single construction.
We performed the ligation finO+vector pSB1A3 for an O/N period, according to protocol Y (see Protocols section).
finP+pSB1A3 ligation
As we did for finO sequence, we also ligated finP to the vector pSB1A3 according to the same protocol. The ligation also lasted O/N period.
Marcelo
YeastGuard
New biobricks in biobrick format
E. coli transformed with the new biobricks grew very well on the LB+AMP plates!
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