The samples from yesterday's electroelution were applied to a 0,8% agarose gel and submitted to 80 V during 30 minutes. A band of approximately 22 kb was formed, indicating that the F plasmid was succesfully purified!
Now that the F plasmid was obtained, the next step is its recircularization.
Gabriel
finO and finP - Still Trying to Confirm our Biobricks
We inoculated those colonies that actually resulted in a expected amplification size fragment after the miniprep procedure (recent assays), separately into liquid LB-AMP medium, in order to repeat the miniprep, aiming in reducing the amount of genomic DNA extracted together.
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