Ligation of finOP and Cre-Recombinase on pGEM vector
In order to start our pGEM strategy on assembling biobricks, today we ligated PCR's products of finO, finP and Cre-Recombinase into pGEM Vector. There is no need for any digestion before this procedure, since pGEM has an T stick ends capable of pairing with the A stick ends left by Taq Polymerase on the PCR products.
We followed protocol 11, without modifications.
Ligation lasted 1 hour.
Marcelo and Victor
Transformation of finOP and Cre-Recombinase ligations
After performing the required ligations, we then transformed them into electrocompetent E. coli bacteria, strain DH10B, according to protocol 3.
We plated the trasformed cells in LB-AMP media, which already contained X-gal substrate.
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