Notebook > AND_Gate_2 > T3
2009.7.30
12:30 T7 Polymerase store the strain
13:00 T3 Promoter + GFP Double digestion check
13:00 T3 Promoter Parts Double digestion check
13:45 T7 Polymerase Miniprep
2009.8.1
15:00 T7 Polymerase + 1-2M check
2009.8.5
18:20 T3 Promoter PCR
2009.8.6
21:30 Gel recycle of T3 Promoter PCR
23:45 T3 Promoter PCR Product Double digestion
2009.8.7
11:00 Purify of T3 Promoter Digested product
11:00 Three Counter Plasmid Miniprep
15:30 T3 Polymerase High pure PCR
18:00 Gel recycle of T3 Polymerase PCR
2009.8.8
11:00 Bi-stable Miniprep
12:00 Bi-stable Plasmid Double Digestion
2009.8.9
21:00 Ligation of T3 Promoter (insert) and PSB1K3 (Vector)
21:15 T3 Polymerase PCR Product Double Digestion
2009.8.10
6:00 T3 Polymerase Digestion Product Purify
6:30 Ligation of T3 Polymerase (insert) and PSB1K3 (Vector)
10:00 Transformation of T3 Promoter
22:00 Transformation of T3 Polymerase
2009.8.11
4:00 Sake the T3 Promoter Parts
4:00 PCR of T3 Promoter Parts
16:30 T3 Promoter Parts Miniprep
2009.8.13
13:30 Miniprep of T3 Polymerase
14:30 Double digestion of T3 Polymerase plasmid
2009.8.15
12:00 Minioreo of T3 Polymerase
14:15 Double digestion of T3 Polymerase plasmid
15:00 Sake the T3 Promoter Parts
20:00 Sake the T3 Polymerase
2009.8.16
14:15 Miniprep of T3 Promoter Parts
16:00 Miniprep of T3 Polymerase
2009.8.17
10:30 Double digestion of T3 Promoter Parts and T3 Polymerase
11:00 Miniprep of 3 Counter Plasmid and T3 Polymerase
22:00 Sake the T3 Polymerase
2009.8.18
12:00 Miniprep of T3 Polymerase
15:00 Double digestion of T3 Polymerase plasmid
2009.8.20
10:00 PCR of T3 Polymerase
14:00 PCR of T3 Promoter + GFP
17:00 PCR of T3 Promoter
20:00 Cut Gel
2009.8.22
12:00 Confirm the PCR product of T3 Promoter
2009.8.23
13:00 Gel cycle of T3 Polymerase and T3 Promoter + GFP
15:00 Double digestion of T3 Polymerase and T3 Promoter + GFP
21:00 Ligation of digested T3 Polymerase and T3 Promoter + GFP
23:00 Transformation of T3 Polymerase and T3 Promoter + GFP
2009.8.25
22:00 Shake the T3 Polymerase and T3 Promoter + GFP
2009.8.26
11:00 Miniprep of the T3 Polymerase and T3 Promoter + GFP
15:00 Double digestion of T3 Polymerase and T3 Promoter + GFP
2009.8.27
0:00 Shake the T3 Polymerase (Strain 3) and T3 Promoter + GFP (Strain 1)
13:00 Miniprep of T3 Polymerase (Strain 3) and T3 Promoter + GFP (Strain 1)
2009.8.28
10:00 Single Point Mutation of T3 Polymerase
20:00 Ligation of Mutated T3 Polymerase
21:00 Transformation of Mutated T3 Polymerase
2009.8.29
12:00 Double digestion of T3 Polymerase (Strain 3) and T3 Promoter + GFP (Strain 1)
16:00 Transformation of CI Promoter Parts
2009.8.30
16:00 Double digestion of T3 Polymerase and T3 Promoter + GFP
20:00 Shanke CI Promoter and 3 Counter
21:00 Cut Gel of T3 Polymerase
2009.9.2
11:30 Gel cycle of T3 Polymerase
14:00 Miniprep of 3 Coumter Plasmid
2009.9.4
12:00 Ligation of T3 Polymerase and 1-2M
16:00 Transformation of T3 Polymerase and 1-2M
20:00 PCR of T3 Polymerase (long primer)
2009.9.6
11:00 Shake the T3 Polymerase + 1-2M
15:00 Cut Gel of PCR Product of T3 Polymerase
15:30 Gel cycle
2009.9.7
11:00 Double digestion of T3 Polymerase PCR Product
11:30 Double digestion of T3 Promoter + GFP
2009.9.11
11:00 Shake 1-1M
12:00 Double digestion of T3 Promoter + GFP (High concerntration)
2009.9.13
12:00 Miniprep of 1-1M
16:00 Gel cycle of Double digestion of T3 Promoter + GFP (High concerntration)
2009.9.14
12:00 Single digestion to ensure the T3 Promoter + GFP Plasmid
16:00 Miniprep of 1-1M
2009.9.16
13:00 Double digestion of 1-1M
2009.9.18
13:00 Double digestion of 1-1M (High Concerntration)
2009.9.20
9:00 Cut Gel of 1-1M
9:15 Gel cycle
11:00 Shake T3 Promoter + GFP
2009.9.21
13:00 Miniprep of T3 Promoter + GFP
16:00 Double digestion of T3 Promoter + GFP
2009.9.22
10:00 Ligation of T3 Polymerase and 1-1M
18:00 Transformation of T3 Polymerase
2009.9.23
20:30 Shake T3 Polymerase +1-1M
^Top
|
"
