Notebook
I)
Results for yeaterday's tranformation.
CBG = Cohesive, Bangalore Genei
BBG = Blunt, Bangalore Genei
CP = Cohesive, Promega
PCR
- NEB 1 Colony
- DH5a ~50 Colonies
- NEB 2 Colonies
- DH5a ~150 Colonies
- NEB 0 Colonies
- ~70 Colonies
- NEB 0 Colonies
- DH5a ~130 Colonies
Hence, DH5a Rocks...
Also, PCR works.
II)
Inoculated a single colony of this to LB+KAN for plasmid isolation.
III)
Yesterdays PCR gave us only 2 bands, other than the expected range.. :-(
Why?
- Wrong template?
- Wrong ladder?
--rerun gel and rerun pcr--
III)
Ran a test PCR of a-Mating Factor, p3w13d
IV)
Double RE digest of Lycopene Cyclase.
Ran a gel.
Great gel, expected result.
V)
Gel Eluted.
- XP - 159ng/ul
- SP - 56 ng/ul
kept for ligation.
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