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Team:Brown/Notebook Meetings/9-11-09
From 2009.igem.org
iGEM Meeting
9/11/09
SFH 218
- 8:00 AM: Gary reminds us of our responsibility. We need to plan this semester!
- These next few weeks are the most important
- Adrian: Deadlines
- Jamboree is in 7 weeks!
- Wiki due date: 6 weeks. Adrian recommends we have it done in 5 weeks
- Presentation practice should be started in 5 weeks
- Data collection should be heavy these next few weeks
- Gary:
- Judging is done by a criteria. Criteria is available to us.
- Regular meetings: Friday 8AM in SFH 218
- Team 1 update
- Did not have any expression on the western blots
- Made new BL21 competent cells, however no transformations
- Test competence of cells
- End of next week goal: protein expression
- Team 2 update
- Made all of the constructs needed
- Agr-GFP ligated
- Trg-EnvZ
- Signal Pep-GFP
- Trouble is transforming into S. epi
- Looking for new vectors – talking to same guy that sent us PLM6
- Primers designed for adapting to new vector
- Will’s computer program worked; designed for Tar
- He is concerned about mutagenesis – time consuming
- Submit for synthesis
- Will will pick 5 candidates
- Team 3 update
- We have OmC-RFP and Taz in pNoTat
- Ashley did stratagene, however her positive control plate did not work
- We can test with aspartate
- Will also test ribose binding protein
- If double transformation does not work, sequential transformation
- Everybody: come up with a week-by-week goal sheet.
- The team is meeting next Tuesday at 8AM.
