Team:UNICAMP-Brazil/Notebooks/August 9

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ColiGuard

Selection of BioBricks

Today we selected the biobricks that we are going to use in the Coliguard subproject. They are listed below.

  • Part:BBa_B0030 - RBS.1 (strong) -- modified from R. Weiss

Spring 2009 Distribution>2009 Kit Plate 1>1H

Spring 2008 Distribution>Source Plate 1002>5G

  • Part:BBa_B0032 - RBS.3 (medium) -- derivative of BBa_0030

Spring 2009 Distribution>2009 Kit Plate 1>2I

Spring 2008 Distribution>Source Plate 1000>2C

  • Part:BBa_J61100 - Ribosome Binding Site Family Member

Spring 2009 Distribution>2009 Kit Plate 1>5J

Spring 2008 Distribution>Source Plate 1003>11F

  • Part:BBa_J61107 - Ribosome Binding Site Family Member

Spring 2009 Distribution>2009 Kit Plate 1>5N

Spring 2008 Distribution>Source Plate 1003>12E

  • Part:BBa_B0010 - T1 from E. coli rrnB

Spring 2009 Distribution>2009 Kit Plate 1>13D

Spring 2008 Distribution>Source Plate 1004>5D

  • Part:BBa_B0014 - double terminator

Spring 2009 Distribution>2009 Kit Plate 2>24C

Spring 2008 Distribution>Source Plate 1016>2E

  • Part:BBa_B0015 - double terminator (B0010-B0012)

Spring 2009 Distribution>2009 Kit Plate 1>23L

Spring 2008 Distribution>Source Plate 1016>5F

  • Part:BBa_R0040 - TetR repressible promoter

Spring 2009 Distribution>2009 Kit Plate 1>6I

Spring 2008 Distribution>Source Plate 1000>4C

  • Part:BBa_J23100 - constitutive promoter family member

Spring 2009 Distribution>2009 Kit Plate 1>18C

Spring 2008 Distribution>Source Plate 1002>10E

  • Part:BBa_J23107 - constitutive promoter family member

Spring 2009 Distribution>2009 Kit Plate 1>20A

Spring 2008 Distribution>Source Plate 1002>11D

  • Part:BBa_J23109 - constitutive promoter family member

Spring 2009 Distribution>2009 Kit Plate 2>2G

Spring 2009 Source Plates>S09 SP 2005>1D

  • Part:BBa_E0040 - green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212)

Spring 2009 Distribution>2009 Kit Plate 1>14K

Spring 2008 Distribution>Source Plate 1001>7E

  • Part:BBa_J31005:Get Part - chloramphenicol acetyltransferase (forwards, CmF)

Spring 2009 Distribution>2009 Kit Plate 1>1P

Spring 2008 Distribution>Source Plate 1002>6C

  • Part:BBa_J31007 - tetracycline resistance protein TetA(C)

Spring 2009 Distribution>2009 Kit Plate 1>1L

Spring 2008 Distribution>Source Plate 1002>6ยช

  • Part:BBa_J31003 - kanamycin resistance forward

Spring 2008 Distribution>Source Plate 1002>6E

Registry 2006>2006 Source Plate 1>3E

  • Part:BBa_E1010: **highly** engineered mutant of red fluorescent protein from Discosoma striata

Spring 2009 Distribution>2009 Kit Plate 1>18F

Spring 2008 Distribution>Source Plate 1013>7G

  • Part:pSB1A3 - High copy BioBrick assembly plasmid

Spring 2009 Distribution 2009 Kit Plate 1 1K

  • Part:pSB1AK3 - High copy BioBrick assembly plasmid

Spring 2009 Distribution2009 Kit Plate 1 13A

  • Part:pSB3K3 - Low to medium copy BioBrick standard vector

Spring 2009 Distribution2009 Kit Plate 17E

  • Part:pSB6A1 - pBR322 converted to BioBrick vector (formally BBa_I739201)

Spring 2009 Distribution2009 Kit Plate 1 1O BBa_J04450





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