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ColiGuard
Cre-Recombinase without ATG
The colonies grew up well! All of them were transparent.
We inoculated twenty colonies in liquid LB-AMP media in order to make a miniprep tomorrow.
We let then grow for overnight at 37°C.
Victor
finOP and Cre-Recombinase with pGEM strategy
Yesterday's transformed and plated cells grew in the media! We found white and blue colonies in the plate just as expected (due to the beta-galactosidase gene contained in pGEM vector).
We selected 10 white colonies (those theorically contains our inserts in the correct position, since it interrupts the coding sequence of beta-galactosidase) from each plate and inoculated them into liquid LB-AMP media.
Inocula were keep at 37ºC, under 250 rpm, for an O/N period.
Fabi, Leo, Marcelo and Victor
Transformation of the BBa K112806 + BBa B0015 ligation
Léo
PY Promoter - Transformation
Fabi and Léo
YeastGuard
Raíssa and Taís
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