Deposited BioBricks.html

From 2009.igem.org

Revision as of 17:08, 21 October 2009 by Mverce (Talk | contribs)



Designed BioBricks

We have designed four NEW functionalised linker-extension standard vectors. All together, 106 parts were generated all cloned into our NEW functionalised linker-extension standard vectors and forty-one were single coding, thirteen intermediates and forty-eight composite parts.

Plasmid backbones

Four NEW linker-extension standard vectors are constructed.


NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005]Plasmid_BackboneBB-NIC-II-HisN2261
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006]Plasmid_BackboneBB-NIC-II-NC2240
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]Plasmid_BackboneBB-NIC-II-HisC2167
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]Plasmid_BackboneBB-NIC-III-HisN2261


The coding sequences

The forty-one BioBrick coding sequences are prepared and are listed as (i) coiled-coil-forming segments, (ii) oligimerisation domains, (iii) functional domains and others.

Coiled-coil segments

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038]CodingNZ - cc homodimer87
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039]CodingCZ90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245118 BBa_K245118]CodingP199
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245119 BBa_K245119]CodingP299
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245120 BBa_K245120]CodingP399
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245121 BBa_K245121]CodingP499
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245122 BBa_K245122]CodingP599
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245123 BBa_K245123]CodingP699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245124 BBa_K245124]CodingP799
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125]CodingP899
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245126 BBa_K245126]CodingH199
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127]CodingGCN99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245132 BBa_K245132]CodingAPH190
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245133 BBa_K245133]CodingAPH135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134]CodingBCR108


Oligomerization domains

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026]CodingTA63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027]CodingTB63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028]CodingTC63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029]CodingT1W_DDD189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030]CodingT1W_KKK189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031]CodingT1W_EEE186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042]CodingVASP135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116]CodingGyrB657
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245117 BBa_K245117]CodingGyrB-SG663
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128]Codingp5390
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129]CodingCutA1333
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245130 BBa_K245130]CodingT1W_AEA186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141]Codingfoldon81


Functional domains

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]CodingcYFP252
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]CodingnYFP462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024]CodingZ136
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025]CodingAUP36
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046]CodingccdB306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113]CodingKSI-DP378
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114]CodingLL37111
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]CodingKSI-DP-P2/33405
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140]CodingmNGF669


Others

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131]CodingELST150
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139]CodingRADA66



The intermediate sequences

Thirteen BioBricks with intermediate parts are constructed.

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048]IntermediateH1-P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049]IntermediateKSI-DP-P1489
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052]IntermediateGCN_P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053]IntermediateP4-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054]IntermediateP2-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055]IntermediateP6-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056]IntermediateP3-P5204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057]IntermediateP4-P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059]IntermediateLL37-ELST267
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060]IntermediateELST-ELST-ELST462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061]IntermediateELST-ELST306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096]IntermediateBCR-P2213
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097]IntermediateAPH-P2240



Composite parts

The forty-eight BioBricks as composites are prepared, which are used in (i) polypeptide manufacturing, (ii) polypeptide membranes, (iii) coiled-coil polyhedra, (iv) regulated assembly and (v) functional biopolymers.


Polypeptide manufacturing

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]CodingKSI-DP-P2/33405


Polypeptide membrane

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245091 BBa_K245091]Compositep53-APH1186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245092 BBa_K245092]Compositep53-APH231
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245093 BBa_K245093]Compositep53-BCR204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245099 BBa_K245099]CompositeAPH-p53231
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245100 BBa_K245100]CompositeBCR-p53204


Coiled-coil polyhedra

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245066 BBa_K245066]CompositeP1-GCN-P2309


Regulated assembly

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245098 BBa_K245098]CompositeGyrB-foldon744
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245050 BBa_K245050]CompositeGyrB-CutA1996
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245051 BBa_K245051]CompositeGyrB-SG-SG-CutA11002


Functional biopolymers

NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245102 BBa_K245102]CompositeLL37-ELST-GCN-ELST-P2633
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245105 BBa_K245105]CompositeELST-ELST-ELST-mNGF1137



Slovenia 2009 iGEM Team Parts Sandbox



NameTypeDescriptionLength
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245005 BBa_K245005]Plasmid_BackboneBB-NIC-II-HisN2261
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245006 BBa_K245006]Plasmid_BackboneBB-NIC-II-NC2240
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245007 BBa_K245007]Plasmid_BackboneBB-NIC-II-HisC2167
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245008 BBa_K245008]Plasmid_BackboneBB-NIC-III-HisN2261
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245009 BBa_K245009]DNARFC 37 Scar Sequence6
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245021 BBa_K245021]CodingcYFP252
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245022 BBa_K245022]CodingnYFP462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245024 BBa_K245024]CodingZ136
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245025 BBa_K245025]CodingAUP36
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245026 BBa_K245026]CodingTA63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245027 BBa_K245027]CodingTB63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245028 BBa_K245028]CodingTC63
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245029 BBa_K245029]CodingT1W_DDD189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245030 BBa_K245030]CodingT1W_KKK189
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245031 BBa_K245031]CodingT1W_EEE186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245038 BBa_K245038]CodingNZ - cc homodimer87
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245039 BBa_K245039]CodingCZ90
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245042 BBa_K245042]CodingVASP135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245046 BBa_K245046]CodingccdB306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245048 BBa_K245048]IntermediateH1-P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245049 BBa_K245049]IntermediateKSI-DP-P1489
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245050 BBa_K245050]CompositeGyrB-CutA1996
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245051 BBa_K245051]CompositeGyrB-SG-SG-CutA11002
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245052 BBa_K245052]IntermediateGCN_P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245053 BBa_K245053]IntermediateP4-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245054 BBa_K245054]IntermediateP2-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245055 BBa_K245055]IntermediateP6-H1204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245056 BBa_K245056]IntermediateP3-P5204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245057 BBa_K245057]IntermediateP4-P2204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245058 BBa_K245058]CompositeKSI-DP-P6489
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245059 BBa_K245059]IntermediateLL37-ELST267
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245060 BBa_K245060]IntermediateELST-ELST-ELST462
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245061 BBa_K245061]IntermediateELST-ELST306
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245062 BBa_K245062]CompositeP1-H1-P2309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245063 BBa_K245063]CompositeKSI-DP-P1-H1-P2699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245064 BBa_K245064]CompositeP1-BCR-P2318
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245065 BBa_K245065]CompositeP1-APH-P2345
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245066 BBa_K245066]CompositeP1-GCN-P2309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245067 BBa_K245067]CompositeP3-P4-H1309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245068 BBa_K245068]CompositeP1-P3-P5309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245069 BBa_K245069]CompositeKSI-DP-P1-P3-P5699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245070 BBa_K245070]CompositeP6-P4-P2309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245071 BBa_K245071]CompositeKSI-DP-P6-P4-P2699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245072 BBa_K245072]CompositeKSI-DP-LL37-ELST657
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245073 BBa_K245073]CompositeKSI-DP-LL37-ELST-ELST-ELST-ELST1125
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245074 BBa_K245074]CompositeKSI-DP-LL-37-ELST-GCN-ELST918
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245075 BBa_K245075]CompositeKSI-DP-EEE576
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245076 BBa_K245076]CompositeKSI_DP-LL37-ELST-P2-ELST918
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245077 BBa_K245077]CompositeKSI-DP-LL37-ELST-P4-ELST918
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245078 BBa_K245078]CompositeLL37-ELST-GCN-ELST528
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245079 BBa_K245079]CompositeGCN-ELST255
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245080 BBa_K245080]DNAscar Xba/Spe NgoMIV12
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245081 BBa_K245081]CompositeP2-ELST255
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245082 BBa_K245082]CompositeP4-ELST255
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245083 BBa_K245083]CompositeTA-P1168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245084 BBa_K245084]CompositeTB-P3168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245085 BBa_K245085]CompositeTC-P5168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245086 BBa_K245086]CompositeP2-TA168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245087 BBa_K245087]CompositeP4-TB168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245088 BBa_K245088]CompositeTC-P5168
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245089 BBa_K245089]Compositefoldon-APH222
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245090 BBa_K245090]Compositefoldon-BCR195
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245091 BBa_K245091]Compositep53-APH1186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245092 BBa_K245092]Compositep53-APH231
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245093 BBa_K245093]Compositep53-BCR204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245094 BBa_K245094]Compositefoldon-p53177
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245095 BBa_K245095]CompositeGyrB-p53753
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245096 BBa_K245096]IntermediateBCR-P2213
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245097 BBa_K245097]IntermediateAPH-P2240
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245098 BBa_K245098]CompositeGyrB-foldon744
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245099 BBa_K245099]CompositeAPH-p53231
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245100 BBa_K245100]CompositeBCR-p53204
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245101 BBa_K245101]CompositeLL37-ELST-GCN-ELST-P1633
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245102 BBa_K245102]CompositeLL37-ELST-GCN-ELST-P2633
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245105 BBa_K245105]CompositeELST-ELST-ELST-mNGF1137
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245106 BBa_K245106]CompositeRADA-mNGF741
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245107 BBa_K245107]CompositeLL37-ELST-GCN-ELST-mNGF1203
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245108 BBa_K245108]CompositeKSI-DP-P2/33-LL37-ELST-GCN-ELST945
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245111 BBa_K245111]CompositeP2-P6-H1309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245112 BBa_K245112]CompositeP5-P2-H1309
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245113 BBa_K245113]CodingKSI-DP378
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245114 BBa_K245114]CodingLL37111
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245115 BBa_K245115]CodingKSI-DP-P2/33405
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245116 BBa_K245116]CodingGyrB657
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245117 BBa_K245117]CodingGyrB-SG663
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245118 BBa_K245118]CodingP199
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245119 BBa_K245119]CodingP299
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245120 BBa_K245120]CodingP399
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245121 BBa_K245121]CodingP499
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245122 BBa_K245122]CodingP599
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245123 BBa_K245123]CodingP699
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245124 BBa_K245124]CodingP799
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245125 BBa_K245125]CodingP899
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245126 BBa_K245126]CodingH199
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245127 BBa_K245127]CodingGCN99
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245128 BBa_K245128]Codingp5390
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245129 BBa_K245129]CodingCutA1333
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245130 BBa_K245130]CodingT1W_AEA186
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245131 BBa_K245131]CodingELST150
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245132 BBa_K245132]CodingAPH190
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245133 BBa_K245133]CodingAPH135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245134 BBa_K245134]CodingBCR108
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245135 BBa_K245135]CodingAPHE135
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245139 BBa_K245139]CodingRADA66
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245140 BBa_K245140]CodingmNGF669
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245141 BBa_K245141]Codingfoldon81
[http://partsregistry.org/wiki/index.php?title=Part:BBa_K245200 BBa_K245200]CompositeP1-P4-H1309



Locations of visitors to this page