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EPF-Lausanne/14 October 2009
From 2009.igem.org
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| - | We redid the initial experiment with these cells: incubated them for about | + | We redid the initial experiment with these cells: incubated them for about 2h30 in 4 different conditions: |
- +light+IPTG-Trp | - +light+IPTG-Trp | ||
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The results are the following: | The results are the following: | ||
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| + | [[Image:141009_dh5_ro2dt_2h30.jpg|center|thumb|upright=4|RO2 double-transformants in DH5-alpha]] | ||
==People in the lab== | ==People in the lab== | ||
Revision as of 15:02, 14 October 2009
Contents |
Wet Lab
Transformation of LOVTAP in iGEM plasmid worked --> Check if the insert is correct with Colony PCR & agarose gel
Also: our negative control worked (no clones grew)
Dh5-alpha RO2.4+BB1 n°3
We redid the initial experiment with these cells: incubated them for about 2h30 in 4 different conditions:
- +light+IPTG-Trp
- -light+IPTG-Trp
- -light-IPTG+Trp
- -light-IPTG-Trp
The results are the following:
People in the lab
Gabriela, Tú
