EPF-Lausanne/14 October 2009

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Wet Lab

Transformation of LOVTAP in iGEM plasmid worked --> Check if the insert is correct with Colony PCR & agarose gel

Also: our negative control worked (no clones grew)

Dh5-alpha RO2.4+BB1 n°3

We redid the initial experiment with these cells: incubated them for about 2h in 4 different conditions:

- +light+IPTG-Trp

- -light+IPTG-Trp

- -light-IPTG+Trp

- -light-IPTG-Trp

The results are the following:

Gabriela, Tú