EPF-Lausanne/25 August 2009

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Contents

25 August 2009




Wet Lab

Miniprep:

Made according to the protocol of Aisgen Miniprep Kit Elution in 50 ul. The RD2 #5 plasmids prep as some that have already been prepared will be sent for sequencing this afternoon.


Mediums

New medium were made to grow the double-transformed DH5 a (LovTap BB and RO2) -> should be without thy.

M9 was redone, pH adjusted with amino acid and filtred. for 1L:

5g glucose
6g Na2PO4
3g KH2PO4
1g NH4Cl
0.5g NaCl
0.12g MgSO4
0.01g CaCl2


Trp Operon synthesis

Using the Klenow fragments protocol and primers:£

Trp operon Rev
Trp operon Fwd
Parts&Characteristics
2x Trp 1x Negative Control

NEB 2 (10x)

2ul each

2ul

[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]

Promoter LacI

A

1D (1)

[http://partsregistry.org/Part:BBa_B0030 BBa_B0030]

RBS

A

1H (1)

[http://partsregistry.org/Part:BBa_E0240 BBa_E0240]

RBS-GFP-TER

A

12M (1)

[http://partsregistry.org/Part:BBa_I13507 BBa_I13507]

RBS-mRFP-TER

A

22O (1)

[http://partsregistry.org/Part:BBa_J13002 BBa_J13002]

pTetR-RBS

A

13B (1)

People in the lab

Nath, Basile, Gab, Christian



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