Team:Brown/Notebook Meetings/6-16-09
From 2009.igem.org
Team Tasks- June 16, 2009
- Team 1: EV131- Indu, Ahmad, Minoo
- Team 2: S.aureus- Eli, Flora, Will
- Team 3: Bistable toggle- Michael, Ashley, Steph
- Tasks on hand:
- Team 1:
- Posted on google groups
- Transformation done→ need to grow them up
- Ordered primers
- Sequence protein?
- Sit down and write down protocols
- Put it in pnotat vector; transform
- Affinity tests- Keq
- Team 2:
- S.aureus→ go to biosafety, find strain, lab regulators today: 164 Angell Street
- TJ Foster→ email reg. signaling tag
- Talk to labs that have used S.aureus
- Quorum kill switch
- Directed evolution of histamine signal transduction prokaryotic gram+ system
- Encapsulation
- Team 3:
- BioBricks are in registry except Pxyl/TetO→ contact TJ Foster (check for sequence)
- Construction of model circuit
- Read Collins paper (methods!!!)