Team:Brown/Notebook Meetings/9-11-09

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Latest revision as of 01:58, 22 October 2009

iGEM Meeting

9/11/09

SFH 218

8:00 AM: Gary reminds us of our responsibility. We need to plan this semester!
- These next few weeks are the most important

Adrian: Deadlines
Jamboree is in 7 weeks!
Wiki due date: 6 weeks. Adrian recommends we have it done in 5 weeks
Presentation practice should be started in 5 weeks
Data collection should be heavy these next few weeks
Gary:
- Judging is done by a criteria. Criteria is available to us.
- Regular meetings: Friday 8AM in SFH 218

Team 1 update
- Did not have any expression on the western blots
- Made new BL21 competent cells, however no transformations
- Test competence of cells
- End of next week goal: protein expression

Team 2 update
- Made all of the constructs needed
- Agr-GFP ligated
- Trg-EnvZ
- Signal Pep-GFP
- Trouble is transforming into S. epi
- Looking for new vectors – talking to same guy that sent us PLM6
- Primers designed for adapting to new vector
- Will’s computer program worked; designed for Tar
- He is concerned about mutagenesis – time consuming
- Submit for synthesis
- Will will pick 5 candidates

Team 3 update
- We have OmC-RFP and Taz in pNoTat
- Ashley did stratagene, however her positive control plate did not work
- We can test with aspartate
- Will also test ribose binding protein
- If double transformation does not work, sequential transformation

Everybody: come up with a week-by-week goal sheet.

The team is meeting next Tuesday at 8AM.

@@ Line 10: / Line 10: @@
-/11/09
+'''iGEM Meeting'''
-iGEM Meeting
-SFH 218
-:00 AM: Gary reminds us of our responsibility. We need to plan this semester!
+'''9/11/09'''
-	These next few weeks are the most important
-Adrian: Deadlines
+'''SFH 218'''
-	Jamboree is in 7 weeks!
-	Wiki due date: 6 weeks. Adrian recommends we have it done in 5 weeks
+*8:00 AM: Gary reminds us of our responsibility. We need to plan this semester!
-	Presentation practice should be started in 5 weeks
+**These next few weeks are the most important
-	Data collection should be heavy these next few weeks
-Gary
+*Adrian: Deadlines
-	Judging is done by a criteria. Criteria is available to us.
+*Jamboree is in 7 weeks!
-	Regular meetings: Friday 8AM in SFH 218
+*Wiki due date: 6 weeks. Adrian recommends we have it done in 5 weeks
-Team 1 update
+*Presentation practice should be started in 5 weeks
-	Did not have any expression on the western blots
+*Data collection should be heavy these next few weeks
-	Made new BL21 competent cells, however no transformations
+*Gary:
-	Test competence of cells
+**Judging is done by a criteria. Criteria is available to us.
-	End of next week goal: protein expression
+**Regular meetings: Friday 8AM in SFH 218
-Team 2 update
-	Made all of the constructs needed
+*Team 1 update
-		Agr-GFP ligated
+**Did not have any expression on the western blots
-		Trg-EnvZ
+**Made new BL21 competent cells, however no transformations
-		Signal Pep-GFP
+**Test competence of cells
-	Trouble is transforming into S. epi
+**End of next week goal: protein expression
-		Looking for new vectors – talking to same guy that sent us PLM6
-		Primers designed for adapting to new vector
+*Team 2 update
-	Will’s computer program worked; designed for Tar
+**Made all of the constructs needed
-		He is concerned about mutagenesis – time consuming
+**Agr-GFP ligated
-		Submit for synthesis
+**Trg-EnvZ
-		Will will pick 5 candidates
+**Signal Pep-GFP
-Team 3 update
+**Trouble is transforming into S. epi
-	We have OmC-RFP and Taz in pNoTat
+**Looking for new vectors – talking to same guy that sent us PLM6
-	Ashley did stratagene, however her positive control plate did not work
+**Primers designed for adapting to new vector
-	We can test with aspartate
+**Will’s computer program worked; designed for Tar
-		Will also test ribose binding protein
+**He is concerned about mutagenesis – time consuming
-	If double transformation does not work, sequential transformation
+**Submit for synthesis
-Everybody: come up with a week-by-week goal sheet.
+**Will will pick 5 candidates
-The team is meeting next Tuesday at 8AM.
+*Team 3 update
+**We have OmC-RFP and Taz in pNoTat
+**Ashley did stratagene, however her positive control plate did not work
+**We can test with aspartate
+**Will also test ribose binding protein
+**If double transformation does not work, sequential transformation
+*Everybody: come up with a week-by-week goal sheet.
+*The team is meeting next Tuesday at 8AM.