Team:Cambridge

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'''The Cambridge [https://2009.igem.org/Main_Page 2009 iGEM] team has created two kits of parts that will facilitate the design and construction of biosensors in the the future.
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Previous iGEM teams have focused on genetically engineering bacteria to respond to novel inputs – for example light, or biologically significant compounds. There is an unmistakable need, therefore, to also develop clear, user-friendly outputs, especially for use with biosensors. The most popular output is the expression of a fluorescent protein, detectable using fluorescence microscopy. But, what if we could simply ''see'' the output with our own eyes?
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Previous iGEM teams have focused on genetically engineering bacterial biosensors by enabling bacteria to respond to novel inputs, especially biologically significant compounds. There is an unmistakable need to also develop devices that can '''1) manipulate input by changing the behaviour of the response of the input-sensitive promoter''', and that can '''2) report a response using clear, user-friendly outputs'''. The most popular output is the expression of a fluorescent protein, detectable using fluorescence microscopy. But, what if we could simply see the output with our own eyes?  
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The Cambridge [https://2009.igem.org/Main_Page 2009 iGEM] team is engineering E. coli to produce different pigments in response to different concentrations of an inducer. Our device is a three part system:
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We successfully characterised a set of transcriptional systems for calibrated output - [https://2009.igem.org/Team:Cambridge/Project/Amplification Sensitivity Tuners]. We also successfully expressed a spectrum of pigments in ''E. coli,'' designing a set of [https://2009.igem.org/Team:Cambridge/Project/Pigments Colour Generators].
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'''Sensor''': Our sensor system is sensitive to different concentrations of an inducer.
 
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'''Threshold device''': The threshold device is responsible for the sensitivity to the inducer, and acts as an "on" switch to maximally activate pigment production once the inducer has reached a threshold.
 
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'''Colour''': Pigment production defice.
 
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Thus, we can develop bacterial output machines.  The concentration of the inducer to which they are exposed determines which strains are activated to produce pigment.
 
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<div class="imagelink_1">[https://2009.igem.org/Team:Cambridge/Project]</div><div class="imagelink_2">[https://2009.igem.org/Team:Cambridge/Project/Amplification]</div><div class="imagelink_3">[https://2009.igem.org/Team:Cambridge/Project/Pigments]</div><div class="imagelink_4">[https://2009.igem.org/Team:Cambridge/ImageGallery/TEAM]</div><div class="imagelink_5">[https://2009.igem.org/Team:Cambridge/Team]</div><div class="imagelink_6">[https://2009.igem.org/Team:Cambridge/ImageGallery]</div>
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==== The E. Chromi Team, in Cambridge's 800th year ====
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[[Image:Cambridgeteamphoto.png | 360px]][[Image:IGEMTSHIRTtrans2.png | 360px]]
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Latest revision as of 00:10, 22 October 2009


E. Chromi

Cambridge Frontpage2.png
RAINBOW.png

The Cambridge 2009 iGEM team has created two kits of parts that will facilitate the design and construction of biosensors in the the future.

Previous iGEM teams have focused on genetically engineering bacterial biosensors by enabling bacteria to respond to novel inputs, especially biologically significant compounds. There is an unmistakable need to also develop devices that can 1) manipulate input by changing the behaviour of the response of the input-sensitive promoter, and that can 2) report a response using clear, user-friendly outputs. The most popular output is the expression of a fluorescent protein, detectable using fluorescence microscopy. But, what if we could simply see the output with our own eyes?

We successfully characterised a set of transcriptional systems for calibrated output - Sensitivity Tuners. We also successfully expressed a spectrum of pigments in E. coli, designing a set of Colour Generators.















The E. Chromi Team, in Cambridge's 800th year

Cambridgeteamphoto.pngIGEMTSHIRTtrans2.png

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