Team:Cambridge/Project/CA03

From 2009.igem.org

(Difference between revisions)
(In vivo expression of our devices in E.coli MG1655)
(In vivo expression of our devices in E.coli MG1655)
Line 16: Line 16:
Our preliminary research indicated that carotenoids production in ''E.coli'' TOP10 was very weak. We decided to use another strain, ''E.coli'' MG1655, for in vivo expression of our devices. The amount of carotenoids produced became visibly higher. Generally, the colour became visible after incubation for about 12 hours.
Our preliminary research indicated that carotenoids production in ''E.coli'' TOP10 was very weak. We decided to use another strain, ''E.coli'' MG1655, for in vivo expression of our devices. The amount of carotenoids produced became visibly higher. Generally, the colour became visible after incubation for about 12 hours.
-
''Lycopene-producing device (constitutive)''
+
'''''Lycopene-producing device (constitutive)'''''
-
{| style="color:#CCC; background-color:#3D5089;" cellpadding="6" cellspacing="0" border="1"
+
{| style="color:#CCC; background-color:#3D5089;" cellpadding="5" cellspacing="0" border="1"
! Agar plate colonies
! Agar plate colonies
! Cell pellet from LB culture
! Cell pellet from LB culture
-
|- style="color:#333; background-color:#FFFFFF;" cellpadding="6" cellspacing="0" border="1"
+
|- style="color:#333; background-color:#FFFFFF;" cellpadding="5" cellspacing="0" border="1"
|[[Image:Cam09_lyco_1.jpg]]
|[[Image:Cam09_lyco_1.jpg]]
|[[Image:Cam09_lyco_2.jpg]]
|[[Image:Cam09_lyco_2.jpg]]

Revision as of 12:07, 21 October 2009


Carotenoids

TESTING AND CHARACTERISATION

In vivo expression of our devices in E.coli MG1655

Our preliminary research indicated that carotenoids production in E.coli TOP10 was very weak. We decided to use another strain, E.coli MG1655, for in vivo expression of our devices. The amount of carotenoids produced became visibly higher. Generally, the colour became visible after incubation for about 12 hours.

Lycopene-producing device (constitutive)

Agar plate colonies Cell pellet from LB culture
Cam09 lyco 1.jpg Cam09 lyco 2.jpg
E.coli MG1655 transformed with K274110 and grown on agar plate overnight. Cell pellet of E.coli MG1655 transformed with K274210 (from 20mL LB culture at 37°C for 24 hours).



Cambridge Sponsor Logo1.pngCambridge Sponsor Logo2.pngCambridge Sponsor Logo3.pngCambridge Sponsor Logo4.pngCambridge Sponsor Logo5.pngCambridge Sponsor Logo8.pngCambridge Sponsor Logo6.pngCambridge Sponsor Logo7.pngCambridge Sponsor Logo9.pngCambridge Sponsor Logo10.pngCambridge Sponsor Logo11.pngCambridge Sponsor Logo12.pngCambridge Sponsor Logo14.pngCambridge Sponsor Logo13.pngCambridge Sponsor Logo15.pngCambridge Sponsor Logo16.pngCambridge Sponsor Logo17.pngCambridge Sponsor Logo18.pngCambridge Sponsor Logo19.pngBmglab.jpg