Categories :

Home Team Sponsors Parts Submitted to Registry Image Gallery Leave a Message!

Project :

Overview Sensitivity Tuner --- Characterisation --- Modelling Colour Generators --- Carotenoids (Orange/Red) --- Melanin (Brown) --- Violacein (Purple/Green) The Future Safety

Notebook :

Week 1 Week 2 Week 3 Week 4 Week 5 Week 6 Week 7 Week 8 Week 9 Week 10

Team Logistics :

Protocols Stock List Research Shared Links and Help

Carotenoids

TESTING AND CHARACTERISATION

In vivo expression of our devices in E.coli MG1655

Our preliminary research indicated that carotenoids production in E.coli TOP10 was very weak. We decided to use another strain, E.coli MG1655, for in vivo expression of our devices. The amount of carotenoids produced became visibly higher. Generally, the colour became visible after incubation for about 12 hours.

Lycopene-producing device (constitutive)

E.coli MG1655 transformed with K274110 and grown on agar plate overnight.