Team:Cambridge/Project/ME02

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Melanin Pigment


Design

Constructing the BioBrick

Biobrick

Our aim is to make the MelA gene into a biobrick as follows:

MelAbiobrick.jpg

In order to do so, we had to remove forbidden restriction sites within the gene using primers and the in-fusion PCR technique. Primers were ordered as shown by the image below, to add the prefix and suffix and change the PstI restriction site from CTGCAG to CTGCAA:

MelA primer map.JPG

The PCR's will be done in the following steps, in order to remove both sites and prepare the gene as a biobrick:

MelA.png

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